High-affinity multivalent wheat germ agglutinin ligands by one-pot click reaction
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چکیده
منابع مشابه
High-affinity multivalent wheat germ agglutinin ligands by one-pot click reaction
A series of six mono-, di-, and trivalent N,N'-diacetylchitobiose derivatives was conveniently prepared by employing a one-pot procedure for Cu(II)-catalyzed diazo transfer and Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) starting from commercially available amines. These glycoclusters were probed for their binding potencies to the plant lectin wheat germ agglutinin (WGA) from Triticum vu...
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General. Solid-phase peptide synthesis was carried out in plastic syringe reactors. Fmocprotected amino acids were obtained from Novabiochem (Läufelfingen, Switzerland) or prepared according to standard procedures. Bovine serum albumin (A7638), biotinylated WGA (L5142), alkaline phosphatase-coupled monoclonal anti biotin antibody (A6561), BCIP/NBT tablets (B5655), peroxidase-labeled WGA (L7017)...
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The inhibition of carbohydrate-protein interactions by tailored multivalent ligands is a powerful strategy for the treatment of many human diseases. Crucial for the success of this approach is an understanding of the molecular mechanisms as to how a binding enhancement of a multivalent ligand is achieved. We have synthesized a series of multivalent N-acetylglucosamine (GlcNAc) derivatives and s...
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A series of tetravalent glycocyclopeptides functionalized with GlcNAc was synthesized using copper(i)-catalysed alkyne-azide cycloaddition, oxime ligation and thiol-ene coupling. The binding ability of these compounds towards wheat germ agglutinin was studied by a competitive ELLA test and ITC experiments. While all compounds were able to inhibit WGA binding to GlcNAc-polymer coated surfaces at...
متن کاملWheat Germ Agglutinin
Procedures for the isolation, purification, and crystallization of wheat germ agglutinin are described. The agglutinin was purified 184-fold to homogeneity from commercial wheat germ lipase. A molecular weight of 23,500 was estimated for the protein by means of sedimentation equilibrium and sodium dodecyl sulfate gel electrophoresis. The agghxtinin is a glycoprotein. Amino acid and carbohydrate...
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ژورنال
عنوان ژورنال: Beilstein Journal of Organic Chemistry
سال: 2012
ISSN: 1860-5397
DOI: 10.3762/bjoc.8.91